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Although N-acetylcysteine (NAC) directly influences the pool of extracellular cystine and intracellular cysteine via a series of plasmatic redox reactions, in order to be effective, intracellular cysteine precursors must be designed to enter erythrocytes rapidly and use high activity enzymes within erythrocytes to liberate cysteine (Whillier et al., 2009
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For generating calibration curve of HT1080 cells, exact same conditions were used as described above
BPC-157 is synthesized using solid-phase peptide synthesis (SPPS) and purified via high-performance liquid chromatography (HPLC) to achieve analytical research standards
Lee, J.-H., Kim, H.-L., Lee, M
