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When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)
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The 10 mL size is the standard laboratory configuration for multi-draw reconstitution applications
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Alpha diversity Several alpha diversity measures were analyzed since bacterial evenness and richness can be incorporated to varying degrees in different measures 41
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